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In Vitro Effects of Blue Laser Light as an Antimicrobial Agent on Microbial-Infected Fibroblast Cells
<u>Francis Obeng Brenya1 and Nicolette Nadene Houreld1
1Laser Research Centre, Faculty of Health Science, University of Johannesburg, Johannesburg, South Africa
Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pyogenes are key pathogens that delay healing and pose challenges due to their antibiotic resistance. Antimicrobial photobiomodulation (aPBM) using blue light (400-470 nm) has been shown to have antibacterial properties; however, its effects on mammalian cells are not well understood. We investigated the effect of blue laser light (470 nm, 82.7 mW/cm2, 10 J/cm2, 2 min) on bacteria-infected BJ-5ta fibroblast cells. BJ-5ta cells were co-cultured for 24 h with each of the three bacterial strains (1.50 x 103 CFU/mL) and then exposed to blue light. Fibroblast cell viability and bacterial colony counts were assessed 24 h post-aPBM. Control cells (0 J/cm2) infected with S. aureus exhibited 95% fibroblast cell viability and increased bacterial counts (1.80 x 105 CFU/mL). Control cells (0 J/cm2) infected with S. pyogenes and P. aeruginosa showed 89.5% fibroblast cell viability, with bacterial counts increasing to 3.00 x 105 and 2.36 x 105 CFU/mL, respectively. In irradiated (10 J/cm2) BJ-5ta cells infected with S. aureus, P. aeruginosa, and S. pyogenes, fibroblast cell viability was 89.2%, 94.6%, and 77.6%, respectively. As compared to the controls, bacterial counts decreased to 1.30 x 105 CFU/mL, 1.35 x 105 CFU/mL, and 1.20 x 105 CFU/mL, respectively. Blue light (470 nm, 82.7 mW/cm2, 10 J/cm2) induced bacterial death while preserving fibroblast cell viability after a single exposure. aPBM has the potential to address the medical challenges associated with infected wounds and open new avenues for future research.
| Apply for student award at which level: | PhD |
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| Consent on use of personal information: Abstract Submission | Yes, I ACCEPT |
