Speaker
Description
Pheophorbide a (PPBa) is a natural compound derived from chlorophyll, and its photophysical and photochemical properties makes it useful as a photosensitizer for photodynamic therapy (PDT). However, PPBa stability in biological environments and its bioavailability are crucial for effective therapy. Nanoparticle formulation of PPBa can improve its solubility and stability. The aim of this study is to make use of liposomal nanocomplex of PPBa as photosensitizer in PDT (at 15 J/cm2 fluency) on A549 spheroid cells. Thin-film hydration method was used for synthe-sis of NPs. Characterization of Lipo@PPBa were carried out using UV-Vis spectroscopy, TEM, SEM, FTIR and DLS. Moreover, cytotoxicity of NPs was evaluated at various concentrations via MTT assay. The IC50 dose was calculated for the evaluation of phototoxic effects under 660 nm laser irradiation at 15 J/cm2. UV-Vis spectroscopy showed a specific peak at 220 nm for lipids and two peaks for PPBa at 400 nm and 670 nm, respectively. TEM and SEM images il-lustrated that the size and shape of NPs were 45 nm and wavy crest, respectively. DLS data showed that the NPs have positive surface charge with zeta potential of 25 mV. MTT assay indicated that IC50 of Lipo@PPBa nanocomplex in PDT was 1 µM, which reduced the cell via-bility to 48%. In conclusion, Lipo@PPBa showed significant phototoxic effects on A549 sphe-roid cells. However, more investigations on targeted therapy using Lipo@PPBa is recommend-ed.
| Apply for student award at which level: | PhD |
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| Consent on use of personal information: Abstract Submission | Yes, I ACCEPT |
